Bacterial I.D Lab

Bacterial I.D Lab

1. To grow bacterial colonies in a solid medium culture dish.
2. To prepare the DNA, we got a bacterial colony, and put it in a micro centrifuge tube then got some digestive buffer and put it in the tube with the bacteria. We then let the tube sit for a while and then put it in a water bath at 100 degrees celcius for a while then it in a centrifuge for a while and put the bacteria from the micro centrifuge tube into a PCR tube.
3. PCR is used to make copies of DNA sequences by annealing the primer to the template, and the synthesis of new strands. This takes less than 2 minutes. Each step is carried out in the same vial.  At the end of a cycle, each piece of DNA in the vial has been duplicated. The cycle can be repeated 30 or more times and each newly synthesized DNA acts a new template. 
4. First set up the micro concentrator column. You add buffer to the column and then add the PCR to the column. You then place the tubes in ice, and then next into the centrifuge. Then you don't need the column anymore, so you can get rid of it. 
5. They identify you by copies/ sequences of DNA being produced. 
6. They end up stopping the sequence at a certain point and then go on to the section. They repeat this same process many, many times.
7. Blast stands for Basic Local Alignment Search Tool.
8. It was pretty easy to find the things mutated in the DNA, and yes it had Bartonella Henselae.