Monday, April 2, 2012

DNA Extraction Lab

I had no clue it was this easy to extract DNA from something! It was a very quick and easy lab that didn't take long at all. First we measured out 1 gram of raw wheat germ into a 50 ml test tube. Then we got 20 ml of hot water and added it to the tube with the wheat  germ and stirred it constantly for about 3 minutes or so. Next we added about 1/4 teaspoon of detergent and stirred it about every minute for 5 minutes, without creating any foam. We then had to add 14 ml of alcohol very slowly at an angle into the test tube so that it would form kind of a separate layer on top of the detergent-water-wheat mix. After setting for a few minutes the DNA started rising and forming into clumpy, stringy, snot like strands in the alcohol level. At the top of the detergent and bottom of the alcohol level is where the DNA started to grow. The detergent did the job of busting up the cells, and as i said before, the alcohol brought up the DNA. DNA dissolves in water, but gets clumpy in alcohol and doesn't really dissolve, so that is why the DNA came up into the alcohol level.

1 comment:

  1. Great work on this post- lots of good details about the technique!

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